一种用于检测人对爱泼斯坦-巴尔病毒膜抗原IgA抗体反应的酶联免疫吸附测定法。
An enzyme-linked immunosorbent assay for the measurement of human IgA antibody responses to Epstein-Barr virus membrane antigen.
作者信息
Durda P J, Sullivan M, Kieff E, Pearson G R, Rabin H
机构信息
E.I. DuPont de Nemours and Co., Medical Products, North Billerica, Mass. 01862.
出版信息
Intervirology. 1993;36(1):11-9. doi: 10.1159/000150317.
An enzyme-linked immunosorbent assay (ELISA) which uses a recombinant truncated form of the Epstein-Barr virus (EBV) membrane antigen gp350/250 has been developed and used to measure human IgA antibody responses to that antigen. From comparisons with conventional immunofluorescence assays (IFA) for measuring IgA antibody responses to EBV viral capsid antigens, the ELISA shows comparable specificity and is approximately 4-fold more sensitive. Since IgA antibodies to EBV indicate a high risk of developing nasopharyngeal carcinoma (NPC), the described ELISA, which is more sensitive and objective than IFA, has potential for use in the diagnosis of NPC and for large-scale screening to identify individuals at risk for the development of this disease.
一种酶联免疫吸附测定（ELISA）已被开发出来，该方法使用重组截短形式的爱泼斯坦-巴尔病毒（EBV）膜抗原gp350/250来测量人对该抗原的IgA抗体反应。通过与用于测量对EBV病毒衣壳抗原的IgA抗体反应的传统免疫荧光测定（IFA）进行比较，ELISA显示出相当的特异性，并且灵敏度大约高4倍。由于针对EBV的IgA抗体表明患鼻咽癌（NPC）的风险很高，所描述的ELISA比IFA更灵敏、更客观，具有用于NPC诊断和大规模筛查以识别有患此病风险个体的潜力。
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