使用合成的汇聚肽文库（或混合表位）评估爱泼斯坦-巴尔病毒（EBV）免疫球蛋白M酶联免疫吸附测定法用于原发性EBV感染的诊断。
Evaluation of an Epstein-Barr virus (EBV) immunoglobulin M enzyme-linked immunosorbent assay using a synthetic convergent peptide library, or mixotope, for diagnosis of primary EBV infection.
作者信息
Tranchand-Bunel D, Gras-Masse H, Bourez B, Dedecker L, Auriault C
机构信息
Laboratoire d'Immunologie Cellulaire, CNRS UMR 8527, Institut Pasteur de Lille, 59019 Lille, France.
出版信息
J Clin Microbiol. 1999 Jul;37(7):2366-8. doi: 10.1128/JCM.37.7.2366-2368.1999.
Abstract
An immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) was developed by using a 24-amino-acid peptide of the 18-kDa Epstein-Barr virus (EBV) viral capsid antigen (VCAp18). IgM detection was increased by 23% by using this antigen. Detection of IgM antibodies to the EBV proteins in the new ELISA was 100% specific and 95% sensitive.
摘要
通过使用18 kDa爱泼斯坦-巴尔病毒（EBV）病毒衣壳抗原（VCAp18）的24个氨基酸肽段，开发了一种免疫球蛋白M（IgM）酶联免疫吸附测定（ELISA）。使用该抗原使IgM检测率提高了23%。新ELISA中针对EBV蛋白的IgM抗体检测特异性为100%，敏感性为95%。
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Evaluation of an Epstein-Barr virus (EBV) immunoglobulin M enzyme-linked immunosorbent assay using a synthetic convergent peptide library, or mixotope, for diagnosis of primary EBV infection.使用合成的汇聚肽文库（或混合表位）评估爱泼斯坦-巴尔病毒（EBV）免疫球蛋白M酶联免疫吸附测定法用于原发性EBV感染的诊断。
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