Kulikovskiĭ A V
Zh Mikrobiol Epidemiol Immunobiol. 1976 Jan(1):132-4.
The author used the spores of B. cereus and of its two mutants (10id -- defective by spore coats, and No. 3 -- DPA-deficient). The mentioned microbes were subjected to the action of vapour (99 degrees), 5% sodium hydroxide solution at a temperature of 50 degreeC, and of 0.25% sodium hypochlorite solution. On the basis of the survival curves it was revealed that a mutant with defective coats possessed the least resistance to the factors under study. On these grounds a conclusion was drawn on the important portective function of the spore coat, and not simply of the presence of DPA, in the mechanism of thermo- and chemical resistance of spores.
作者使用了蜡样芽孢杆菌及其两个突变体(10id - 芽孢外壳缺陷型,3号 - 缺二吡啶甲酸型)的孢子。将上述微生物置于蒸汽(99摄氏度)、50摄氏度的5%氢氧化钠溶液以及0.25%次氯酸钠溶液中作用。根据存活曲线发现,外壳有缺陷的突变体对所研究的因素抵抗力最弱。基于此得出结论:在孢子的耐热和耐化学性机制中,芽孢外壳具有重要的保护功能,而不仅仅是二吡啶甲酸的存在起作用。