Friedrich T, Weidner U, Nehls U, Fecke W, Schneider R, Weiss H
Heinrich-Heine-Universität Düsseldorf, Institut für Biochemie, Germany.
J Bioenerg Biomembr. 1993 Aug;25(4):331-7. doi: 10.1007/BF00762458.
The NADH:ubiquinone oxidoreductase (complex I) is made up of a peripheral part and a membrane part. The two parts are arranged perpendicular to each other and give the complex an unusual L-shaped structure. The peripheral part protrudes into the matrix space and constitutes the proximal segment of the electron pathway with the NADH-binding site, the FMN and at least three iron-sulfur clusters. The membrane part constitutes the distal segment of the electron pathway with at least one iron-sulfur cluster and the ubiquinone-binding site. Both parts are assembled separately and relationships of the major structural modules of the two parts with different bacterial enzymes suggest, that both parts also emerged independently in evolution. This assumption is further supported by the conserved order of bacterial complex I genes, which correlates with the topological arrangement of the corresponding subunits in the two parts of complex I.
泛醌氧化还原酶（复合体I）由一个外周部分和一个膜部分组成。这两个部分相互垂直排列，赋予该复合体一个不同寻常的L形结构。外周部分伸入基质空间，并与NADH结合位点、FMN以及至少三个铁硫簇构成电子传递途径的近端部分。膜部分与至少一个铁硫簇和泛醌结合位点构成电子传递途径的远端部分。这两个部分分别组装，并且这两个部分的主要结构模块与不同细菌酶的关系表明，这两个部分在进化中也是独立出现的。细菌复合体I基因的保守顺序进一步支持了这一假设，该顺序与复合体I两个部分中相应亚基的拓扑排列相关。
