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Reversed-phase and ion-pair separations of nucleotides, nucleosides and nucleobases: analysis of biological samples in health and disease.

作者信息

Werner A

机构信息

Sandoz Pharma Ltd., Basle, Switzerland.

出版信息

J Chromatogr. 1993 Aug 25;618(1-2):3-14. doi: 10.1016/0378-4347(93)80024-x.

DOI:10.1016/0378-4347(93)80024-x
PMID:8227262
Abstract

Methods for the assay of nucleotides, nucleosides and nucleobases in biological samples in health and disease are reviewed, with emphasis on reversed-phase and ion-pair reversed-phase techniques for their determination. Modes of extraction from biological samples are discussed with respect of the determination of in vivo concentrations. Advantages and limitations of ion-pair reversed-phase chromatography are discussed with examples from biochemistry and clinical chemistry. The capacity of the high-performance capillary electrophoresis is compared with that of ion-pair reversed-phase chromatography.

摘要

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