De la Fuente M, Del Rio M, Hernanz A
Departamento de Fisiología Animal, Facultad de C. Biológicas, Universidad Complutense, Madrid, Spain.
J Neuroimmunol. 1993 Nov-Dec;48(2):143-50. doi: 10.1016/0165-5728(93)90186-3.
Bombesin and the two mammalian bombesin-related peptides, gastrin-releasing peptide (GRP) and neuromedin C, at physiological concentrations ranging from 10(-11) M to 10(-9) M have been shown in this study to significantly stimulate in vitro the antibody-dependent cellular cytotoxicity (ADCC) and natural killer (NK) activities in BALB/c mouse leukocytes from axillary nodes, spleen and thymus. The three neuropeptides studied induced no change in interleukin-2 production. In addition, these neuropeptides induced in leukocytes from axillary nodes a rapid, transient and significant decrease of intracellular cyclic AMP at 30 s, but a significant transient increase of inositol 1,4,5-trisphosphate levels at 30 and 60 s and a stimulation of protein kinase C activity in membrane fractions after 5 min incubation. These results suggest that inositol phospholipid signalling and cAMP messenger systems are involved in the increase of NK and ADCC activities when leukocytes are incubated in the presence of bombesin, GRP or neuromedin C.
本研究表明，胃泌素释放肽（GRP）和神经介素C这两种与哺乳动物蛙皮素相关的肽以及蛙皮素，在10⁻¹¹M至10⁻⁹M的生理浓度下，可显著刺激来自腋窝淋巴结、脾脏和胸腺的BALB/c小鼠白细胞的抗体依赖性细胞毒性（ADCC）和自然杀伤（NK）活性。所研究的这三种神经肽对白细胞介素-2的产生没有影响。此外，这些神经肽可在30秒时使腋窝淋巴结白细胞中的细胞内环磷酸腺苷迅速、短暂且显著降低，但在30秒和60秒时可使肌醇1,4,5-三磷酸水平显著短暂升高，并在孵育5分钟后刺激膜组分中的蛋白激酶C活性。这些结果表明，当白细胞在蛙皮素、GRP或神经介素C存在的情况下孵育时，肌醇磷脂信号传导和环磷酸腺苷信使系统参与了NK和ADCC活性的增强。
