Del Rio M, De la Fuente M
Departamento de Fisiología Animal, Universidad Complutense, Madrid, Spain.
Regul Pept. 1995 Dec 14;60(2-3):159-66. doi: 10.1016/0167-0115(95)00125-5.
Bombesin and the two mammalian bombesin-related peptides, gastrin-releasing peptide (GRP) and neuromedin C, at physiological concentrations have been previously shown to stimulate significantly in vitro the antibody-dependent cellular cytotoxicity (ADCC) and natural killer (NK) activities in BALB/c mouse leukocytes from axillary nodes, spleen and thymus. In the present work we have shown that adherent cells are required in leukocyte samples for stimulation of cytotoxicity by the neuropeptides, which suggests that this effect may be mediated by those cells. Here we demonstrate the specificity of the effects by reversing them in the presence of the bombesin-antagonist (Leu13-psi CH2NH-Leu14)-BN, and by detecting specific receptors for GRP on macrophages of high and low affinity. Using the same binding technics, no receptors for this neuropeptide were found in non-adherent leukocytes.
蛙皮素以及两种与哺乳动物蛙皮素相关的肽，即胃泌素释放肽（GRP）和神经介素C，在生理浓度下，先前已显示能在体外显著刺激来自腋窝淋巴结、脾脏和胸腺的BALB/c小鼠白细胞中的抗体依赖性细胞毒性（ADCC）和自然杀伤（NK）活性。在本研究中，我们发现白细胞样本中的贴壁细胞是神经肽刺激细胞毒性所必需的，这表明这种效应可能是由这些细胞介导的。在此，我们通过在蛙皮素拮抗剂（Leu13-psi CH2NH-Leu14）-BN存在下逆转这些效应，并检测高亲和力和低亲和力巨噬细胞上GRP的特异性受体，来证明这些效应的特异性。使用相同的结合技术，在非贴壁白细胞中未发现该神经肽的受体。
