Czuppon A B, Chen Z, Rennert S, Engelke T, Meyer H E, Heber M, Baur X
Department of Allergology, Ruhr-University of Bochum, Germany.
J Allergy Clin Immunol. 1993 Nov;92(5):690-7. doi: 10.1016/0091-6749(93)90012-5.
Allergy to latex-containing articles is becoming more and more important because it can result in unexpected life-threatening anaphylactic reactions in sensitized individuals.
A protein of 58 kd with an isoelectric point of 8.45 was purified from raw latex and from latex gloves and identified as the major allergen, completely blocking specific IgE antibodies in the serum of latex-sensitized subjects. The allergen is a noncovalent homotetramer molecule, in which the 14.6 kd monomer was identified, by amino acid composition and sequence homologies of tryptic peptides, to be the rubber elongation factor found in natural latex of the Malaysian rubber tree.
Competitive immunoinhibition tests showed that the starch powder covering the finished gloves is the airborne carrier of the allergen, resulting in bronchial asthma on inhalation. The purified allergen can induce allergic reactions in the nanogram range.
The identification of the allergen (Hev b I) may help to eliminate it during the production of latex-based articles in the future.
对含乳胶制品的过敏正变得越来越重要，因为它会在致敏个体中引发意想不到的危及生命的过敏反应。
从生胶乳和乳胶手套中纯化出一种等电点为8.45的58kd蛋白质，鉴定其为主要过敏原，能完全阻断乳胶致敏受试者血清中的特异性IgE抗体。该过敏原是一种非共价同源四聚体分子，通过胰蛋白酶肽段的氨基酸组成和序列同源性鉴定出14.6kd的单体是马来西亚橡胶树天然胶乳中发现的橡胶延长因子。
竞争性免疫抑制试验表明，成品手套上覆盖的淀粉粉末是过敏原的空气传播载体，吸入后会导致支气管哮喘。纯化的过敏原在纳克范围内就能引发过敏反应。
过敏原（Hev b I）的鉴定可能有助于未来在乳胶制品生产过程中消除它。
