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Reduced binding capacity of concanavalin A-sepharose after treatment with chaotropic agents.

作者信息

Fountoulakis M, Juranville J F

机构信息

F. Hoffmann-La Roche Ltd., Pharmaceutical Research-New Technologies, Department of Biology, Basel, Switzerland.

出版信息

J Biochem Biophys Methods. 1993 Sep;27(2):127-32. doi: 10.1016/0165-022x(93)90056-t.

DOI:10.1016/0165-022x(93)90056-t
PMID:8227942
Abstract

Affinity chromatography on Concanavalin A-Sepharose is widely used for purification of glycoproteins carrying mannose and glucose oligosaccharide moieties. High concentrations of chaotropes elute Concanavalin A from the gel matrix and significantly reduce the binding capacity of the column.

摘要

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