Dong W X, Schneider J, Lacolley P, Brisac A M, Safar M, Cuche J L
Department of Medicine, INSERM (U 337), Faculté de Médecine Broussais, Hôtel-Dieu, Paris, France.
J Auton Nerv Syst. 1993 Aug-Sep;44(2-3):109-17. doi: 10.1016/0165-1838(93)90023-n.
Pre-synaptic endings of the sympathetic nervous fibers control the metabolism of catecholamines, particularly inactivating norepinephrine after its neuronal recapture. The present study was carried out to investigate this segment of the metabolism of catecholamines through measurements of DHPG, DOMA and DOPAC concentrations in plasma. A sensitive and specific radio-enzymatic assay was developed of which the major characteristic is to include the plasma sample in the incubation mixture without initial extraction of the deaminated metabolites. In the rat, there was a statistically significant correlation between norepinephrine and DHPG in both anesthetized and conscious conditions and after clonidine or guanethidine induced reduction of sympathetic activity; thus it can be suggested that plasma DHPG is a good index of neuronal metabolism of norepinephrine in this animal. In humans, our data indicate an interesting correlation between norepinephrine and DOMA concentrations in plasma in resting conditions and within three hours after clonidine. Further studies need to be carried out to establish whether DOMA is a better index of neuronal metabolism of norepinephrine than DHPG.
交感神经纤维的突触前末梢控制着儿茶酚胺的代谢，尤其是在神经元重摄取去甲肾上腺素后使其失活。本研究旨在通过测量血浆中3,4-二羟基苯乙二醇（DHPG）、3,4-二羟基扁桃酸（DOMA）和3,4-二羟基苯乙酸（DOPAC）的浓度来研究儿茶酚胺代谢的这一环节。开发了一种灵敏且特异的放射酶分析法，其主要特点是在孵育混合物中加入血浆样本，而无需预先提取脱氨基代谢产物。在大鼠中，无论是在麻醉状态还是清醒状态下，以及在可乐定或胍乙啶诱导交感神经活性降低后，去甲肾上腺素与DHPG之间均存在统计学上的显著相关性；因此，可以认为血浆DHPG是该动物去甲肾上腺素神经元代谢的良好指标。在人类中，我们的数据表明，在静息状态下以及可乐定给药后三小时内，血浆中去甲肾上腺素与DOMA浓度之间存在有趣的相关性。需要进一步开展研究，以确定DOMA是否比DHPG更能作为去甲肾上腺素神经元代谢的指标。
