Kohsaka K, Matsuoka M, Hirata T, Nakamura M
National Institute for Leprosy Research, Tokyo, Japan.
Int J Lepr Other Mycobact Dis. 1993 Sep;61(3):415-20.
Although the viability of Mycobacterium leprae suspended in distilled water with or without 10% fetal calf serum was reduced approximately 10(-2) to 10(-4) from that of the starting material during the process of lyophilization, bacilli capable of multiplication in nude mouse foot pads were found in the lyophilized samples stored for 4 years at 4 degrees C. The multiplication rate of the lyophilized bacilli which were suspended in 10% serum-water was much higher than that of the bacilli suspended in water only. On the other hand, no reduction of the viability of M. leprae suspended in 10% skim milk-water was demonstrated during the process of lyophilization as well as storage for 2 years at 4 degrees C. From the results obtained here, it could be suggested that M. leprae might be preserved in vitro by means of lyophilized M. leprae was extremely stable during cryopreservation when the bacilli were suspended in 10% skim milk-water. Therefore, the composition of the solution for suspending the bacilli is definitely critical for the maintenance of M. leprae viability by means of lyophilization.
尽管在冻干过程中，悬浮于含或不含10%胎牛血清的蒸馏水中的麻风分枝杆菌的活力相较于起始材料降低了约10⁻²至10⁻⁴，但在4℃下储存4年的冻干样本中发现了能够在裸鼠脚垫中繁殖的杆菌。悬浮于10%血清-水中的冻干杆菌的繁殖率远高于仅悬浮于水中的杆菌。另一方面，在冻干过程以及在4℃下储存2年期间，未证明悬浮于10%脱脂乳-水中的麻风分枝杆菌的活力有所降低。根据此处获得的结果，可以推测麻风分枝杆菌可能通过冻干得以在体外保存，当杆菌悬浮于10%脱脂乳-水中时，冻干的麻风分枝杆菌在冷冻保存期间极其稳定。因此，用于悬浮杆菌的溶液组成对于通过冻干维持麻风分枝杆菌的活力绝对至关重要。
