Rootman D S, Krajden M
Department of Ophthalmology, University of Toronto, Ontario, Canada.
J Ocul Pharmacol. 1993 Fall;9(3):271-6. doi: 10.1089/jop.1993.9.271.
We have previously shown the pharmacokinetic value of delivering gentamicin to the rabbit anterior segment using the Morgan Therapeutic Lens. The present study utilized an intrastromal injection model of Pseudomonas keratitis to test the therapeutic efficacy of continuous flow delivery of gentamicin with the Morgan therapeutic lens. All eyes (n = 52) received an intrastromal injection of approximately 1800 colony forming units (CFU) of Pseudomonas aeruginosa. At 22 hours after injection, eyes were perfused for 6 hours with saline or gentamicin (1, 2.5 or 5 mg/ml), or received gentamicin drops (13.6 mg/ml) at 15 minutes for four doses, then hourly for 6 hours. Corneas were homogenized and plated to determine bacterial survival, and expressed as log colonies (CFU). Log CFU recovered were 7.37 +/- 0.04, 6.64 +/- 0.20, 5.64 +/- 0.31, and 3.56 +/- 0.50 log CFU for saline perfusion, 1, 2.5, 5 mg/ml gentamicin perfusion respectively. Following six hours of treatment with topical fortified gentamicin drops, 5.93 +/- 0.34 log CFU were recovered. Gentamicin perfusion (5 mg/ml) was significantly different from saline or the other treatment groups (P < 0.05). Continuous corneal perfusion with the Morgan Therapeutic Lens demonstrated an increasing dose response curve with increasing perfusate concentration. It was effective in the treatment of experimental Pseudomonas keratitis.
我们之前已经展示了使用摩根治疗性镜片将庆大霉素递送至兔眼前节的药代动力学值。本研究利用铜绿假单胞菌角膜炎的基质内注射模型，来测试摩根治疗性镜片持续流动递送庆大霉素的治疗效果。所有眼睛（n = 52）均接受了约1800个铜绿假单胞菌菌落形成单位（CFU）的基质内注射。注射后22小时，眼睛用生理盐水或庆大霉素（1、2.5或5 mg/ml）灌注6小时，或在15分钟时接受庆大霉素滴眼液（13.6 mg/ml），共四剂，然后每小时一剂，持续6小时。将角膜匀浆并接种平板以确定细菌存活情况，并以对数菌落（CFU）表示。生理盐水灌注、1、2.5、5 mg/ml庆大霉素灌注后回收的对数CFU分别为7.37±0.04、6.64±0.20、5.64±0.31和3.56±0.50 log CFU。用强化庆大霉素滴眼液治疗6小时后，回收的对数CFU为5.93±0.34。庆大霉素灌注（5 mg/ml）与生理盐水或其他治疗组有显著差异（P < 0.05）。使用摩根治疗性镜片进行角膜持续灌注显示，随着灌注液浓度增加，剂量反应曲线上升。它对实验性铜绿假单胞菌角膜炎的治疗有效。
