Falcinelli C, van Belkum A, Schrauwen L, Seldenrijk K, Quint W G
Diagnostic Centre SSDZ, Department of Molecular Biology, Delft, The Netherlands.
J Med Virol. 1993 Aug;40(4):261-5. doi: 10.1002/jmv.1890400402.
By a combination of reversed transcription and subsequent polymerase chain reaction (RNA-PCR), 23 cytologically normal cervical scrapings, positive for the presence of human papillomavirus type 16 (HPV 16) DNA, were analyzed for the presence of transcripts originating from the E6 region of the viral genome. This region is thought to be involved in transformational, tumorigenic events. No mRNAs of the E6 region were detectable using the most sensitive PCR-mediated procedure currently available. Since it was previously shown that in cytological abnormal cervical scrapings about one-half of the samples positive for HPV 16 DNA express mRNAs of the E6 region, a difference between normal and abnormal cervical scrapings, when the HPV 16 is present, exists. The observed difference between cytologically normal and abnormal, HPV-DNA-positive cervical scrapes may eventually be used as a prognostic marker for screening of women at risk for the development of cervical carcinoma. However, firm establishment of the putative correlation between tumor progression and the presence of E6 transcripts requires extensive follow-up analysis of HPV-positive patients.
通过逆转录和随后的聚合酶链反应（RNA-PCR）相结合的方法，对23份细胞学正常且人乳头瘤病毒16型（HPV 16）DNA呈阳性的宫颈刮片进行分析，以检测源自病毒基因组E6区域的转录本。该区域被认为与转化、致瘤事件有关。使用目前最灵敏的PCR介导程序未检测到E6区域的mRNA。因为之前已表明，在细胞学异常的宫颈刮片中，约一半HPV 16 DNA呈阳性的样本表达E6区域的mRNA，所以当存在HPV 16时，正常和异常宫颈刮片之间存在差异。在细胞学正常和异常、HPV-DNA呈阳性的宫颈刮片中观察到的差异最终可能用作筛查有患宫颈癌风险女性的预后标志物。然而，要确定肿瘤进展与E6转录本存在之间的假定相关性，需要对HPV阳性患者进行广泛的随访分析。
