Ngan H Y, Stanley M, Liu S S, Ma H K
Department of Obstetrics and Gynaecology, University of Hong Kong, Queen Mary Hospital.
Genitourin Med. 1994 Jun;70(3):167-70. doi: 10.1136/sti.70.3.167.
To determine the prevalence of HPV 16 and 18 E6 by DNA detection and p53 abnormal protein expression in cervical cancers in Hong Kong.
Seventy-three squamous cell cervical cancer biopsy were analysed. Detection of HPV DNA was carried out by the polymerase chain reaction and Southern blotting (PCR/SB) technique using primers to the HPV16 & 18 E6 region and consensus primers to the L1 region. Abnormal expression of the p53 protein was detected by immunohistochemical staining (IHS) using the antibody CM1 on frozen sections of 55 cervical cancer samples. Forty-six samples were analysed for both the presence of HPV DNA and abnormal expression of p53.
67.2% of the 64 samples showed the presence of HPV 16 E6 DNA and 39.1% showed the presence of HPV 18 E6 DNA. 32.8% showed the presence of both HPV 16 and 18 E6 DNA. No HPV DNA was shown in 10.9% of samples. Only 3.6% (2) of 55 samples showed positive IHS with CM1. One occurred in a HPV negative sample and the other in a HPV positive sample.
A high prevalence of HPV DNA was detected in cervical cancer in Hong Kong using the PCR/SB technique. However, abnormal expression of p53 was uncommon amongst patients with or without HPV 16 or 18 infection.
通过DNA检测以及宫颈癌中p53异常蛋白表达来确定香港地区宫颈癌中HPV 16和18 E6的流行情况。
对73例宫颈鳞状细胞癌活检标本进行分析。采用聚合酶链反应和Southern印迹（PCR/SB）技术，使用针对HPV16和18 E6区域的引物以及针对L1区域的通用引物检测HPV DNA。使用抗体CM1对55例宫颈癌样本的冰冻切片进行免疫组织化学染色（IHS），检测p53蛋白的异常表达。对46个样本同时分析HPV DNA的存在情况和p53的异常表达。
64个样本中67.2%显示存在HPV 16 E6 DNA，39.1%显示存在HPV 18 E6 DNA。32.8%显示同时存在HPV 16和18 E6 DNA。10.9%的样本未显示HPV DNA。55个样本中仅有3.6%（2个）CM1免疫组织化学染色呈阳性。其中1个出现在HPV阴性样本中，另1个出现在HPV阳性样本中。
使用PCR/SB技术检测到香港地区宫颈癌中HPV DNA的高流行率。然而，无论有无HPV 16或18感染，p53的异常表达在患者中并不常见。
