Kann M, Köchel H G, Uy A, Thomssen R
Department of Medical Microbiology, University of Göttingen, Federal Republic of Germany.
J Med Virol. 1993 Aug;40(4):285-90. doi: 10.1002/jmv.1890400406.
The prevalence and time course of the occurrence of antibodies to the hepatitis B virus polymerase (anti-HBpol) were investigated in acutely and in chronically HBV-infected individuals by using recombinant HBpol protein for Western blot analysis. One group consisted of 19 patients who were acutely infected and recovered completely. Five of these patients (26%, 69 serum samples examined) exhibited anti-HBpol. Among those anti-HBpol positive patients, recovery from the disease was combined with a complete loss of this antibody. In contrast, in a second group of 15 individuals who developed chronic hepatitis B, 13 (87%, 102 serum samples examined) had anti-HBpol during the acute phase of the disease. The difference between the anti-HBpol prevalence rates of the two patient groups is statistically significant (Exact Fisher test, P < .002), implying that the occurrence of anti-HBpol may be indicative of a potential chronic course of hepatitis B. Remarkably, anti-HBpol was found in one case of a clinically suspected hepatitis B in which no other serological HBV parameters were found. This serum sample was positive in HBV PCR, supporting a possible diagnostic value of anti-HBpol.
通过使用重组乙肝病毒聚合酶（HBpol）蛋白进行蛋白质印迹分析，研究了急性和慢性乙肝病毒（HBV）感染个体中乙肝病毒聚合酶抗体（抗-HBpol）的流行情况和出现的时间过程。一组由19例急性感染并完全康复的患者组成。其中5例患者（26%，共检测69份血清样本）出现抗-HBpol。在这些抗-HBpol阳性患者中，疾病康复伴随着该抗体的完全消失。相比之下，在第二组15例发展为慢性乙型肝炎的个体中，13例（87%，共检测102份血清样本）在疾病急性期有抗-HBpol。两组患者抗-HBpol流行率的差异具有统计学意义（精确Fisher检验，P < .002），这意味着抗-HBpol的出现可能预示着乙型肝炎潜在的慢性病程。值得注意的是，在1例临床疑似乙型肝炎病例中发现了抗-HBpol，而该病例未发现其他血清学乙肝病毒参数。该血清样本的HBV PCR检测呈阳性，支持抗-HBpol可能具有诊断价值。
