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红细胞与极化电极的相互作用:长程分子间力的证据。

Interaction of red blood cells with a polarized electrode: evidence of long-range intermolecular forces.

作者信息

Gingell D, Fornes J A

出版信息

Biophys J. 1976 Oct;16(10):1131-53. doi: 10.1016/S0006-3495(76)85763-3.

Abstract

We have investigated the electrostatic interaction of glutaraldehyde-fixed human red cells with a polarizable electrode carrying a defined surface charge density which can be varied continuously through a wide range. Cells in a dilute salt solution are unable to adhere to the electrode at high negative charge, but at lower negative charge densities they are reversibly adherent and can be forced off by increasing the negative polarization. Near zero electrode charge they become irreversibly stuck to the electrode and cannot be evicted even at maximum electrode polarization. Calculation of the electrostatic repulsive force using measured charge densities indicates the existence of an attractive force which may be acting over several hundred angstroms.

摘要

我们研究了戊二醛固定的人红细胞与带有可在很宽范围内连续变化的特定表面电荷密度的可极化电极之间的静电相互作用。在稀盐溶液中的细胞在高负电荷时无法附着在电极上,但在较低的负电荷密度下它们会可逆地附着,并且可以通过增加负极化将其推开。在电极电荷接近零时,它们会不可逆地粘附在电极上,即使在最大电极极化时也无法被驱离。使用测量的电荷密度计算静电排斥力表明存在一种可能作用于数百埃的吸引力。

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本文引用的文献

1
Computed force and energy of membrane interaction.
J Theor Biol. 1971 Jan;30(1):121-49. doi: 10.1016/0022-5193(71)90041-5.
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Studies on cellular adhesion in tissue culture. IX. Electrophoretic mobility and contact phenomena.
Exp Cell Res. 1968 Aug-Sep;51(2-3):609-25. doi: 10.1016/0014-4827(68)90149-3.
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