Mahata M, Hörtnagl H, Mahata S K, Fischer-Colbrie R, Winkler H
Department of Pharmacology, University of Innsbruck, Austria.
J Neurochem. 1993 Nov;61(5):1648-56. doi: 10.1111/j.1471-4159.1993.tb09799.x.
The mRNA levels of secretogranin II, chromogranin B, and VGF were compared in brains of control and AF64A-treated rats. This toxin induces specific lesions of the septohippocampal cholinergic pathway. As a consequence of this treatment, the chromogranin B message was elevated in the dentate gyrus granule cells of the hippocampus. In the paraventricular nucleus of the hypothalamus, a concomitant elevation of the messages of secretogranin II and corticotropin-releasing factor occurred in the parvocellular neurons, and an increase of those of secretogranin II and VGF occurred in a subgroup of magnocellular neurons. Further increases for secretogranin II were seen in the amygdaloid nuclei and the reticular thalamic nuclei and increases for chromogranin B in the temporal cortex, substantia nigra compacta, and ventral tegmental area. These results indicate that the toxin-induced lesion of the cholinergic pathway innervating the hippocampus apparently leads to the stimulation of several defined groups of neurons that react with an increase in the mRNA levels of their secretory peptides. We suggest that changes in mRNA expression of these peptides are useful parameters for defining neurons under chronic stimulation.
比较了对照组和经AF64A处理的大鼠大脑中分泌粒蛋白II、嗜铬粒蛋白B和VGF的mRNA水平。这种毒素会诱导海马胆碱能隔区通路的特定损伤。经此处理后，海马齿状回颗粒细胞中的嗜铬粒蛋白B信息升高。在下丘脑室旁核中，小细胞神经元中分泌粒蛋白II和促肾上腺皮质激素释放因子的信息同时升高，在大细胞神经元的一个亚组中，分泌粒蛋白II和VGF的信息增加。在杏仁核和丘脑网状核中，分泌粒蛋白II进一步增加，在颞叶皮质、黑质致密部和腹侧被盖区，嗜铬粒蛋白B增加。这些结果表明，毒素诱导的支配海马的胆碱能通路损伤显然导致了几组特定神经元的刺激，这些神经元通过其分泌肽mRNA水平的增加做出反应。我们认为，这些肽的mRNA表达变化是定义慢性刺激下神经元的有用参数。
