Mahata S K, Mahata M, Hörtnag H, Fischer-Colbrie R, Steiner H J, Dietze O, Winkler H
Department of Pharmacology, University of Innsbruck, Austria.
J Neuroendocrinol. 1993 Jun;5(3):323-30. doi: 10.1111/j.1365-2826.1993.tb00489.x.
In situ hybridization was used to study the mRNA levels for secretogranin II and VGF in comparison with those of oxytocin and vasopressin in the hypothalamus of rats. VGF is a widespread constituent of large dense core vesicles which is selectively induced in PC12 cells by nerve growth factor. After adrenalectomy the mRNA levels of secretogranin II, VGF and vasopressin were increased 4- to 5-fold in the parvocellular neurons of the paraventricular nuclei. In lactating rats the message for oxytocin and secretogranin II were significantly elevated in the magnocellular neurons of the paraventricular and supraoptic nuclei, whereas for VGF only a smaller non-significant increase was observed. As shown by immunoelectron microscopy secretoneurin (a peptide derived from secretogranin II) and oxytocin are co-stored in the large dense core vesicles of the hypothalamo-neurohypophysial neurons. These results demonstrate that stimulation of both parvo- and magnocellular neurons of the hypothalamus induces a concomitant increase of the messages for secretogranin II and VGF together with those of vasopressin and oxytocin.
采用原位杂交技术研究大鼠下丘脑分泌粒蛋白II和VGF的mRNA水平，并与催产素和血管加压素的mRNA水平进行比较。VGF是大致密核心囊泡的一种广泛成分，在神经生长因子的作用下，PC12细胞中可选择性诱导其表达。肾上腺切除术后，室旁核小细胞神经元中分泌粒蛋白II、VGF和血管加压素的mRNA水平增加了4至5倍。在泌乳大鼠中，室旁核和视上核大细胞神经元中催产素和分泌粒蛋白II的信息显著升高，而VGF仅观察到较小的非显著性增加。免疫电子显微镜显示，分泌神经肽（一种源自分泌粒蛋白II的肽）和催产素共同储存在下丘脑-神经垂体神经元的大致密核心囊泡中。这些结果表明，下丘脑小细胞和大细胞神经元的刺激会导致分泌粒蛋白II和VGF的信息与血管加压素和催产素的信息同时增加。
