Worcester D L
Brookhaven Symp Biol. 1976 May(27):III37-III57.
In this paper the methods and techniques of neutron diffraction studies of biological membranes and membrane components have been described and demonstrated. Foremost among these are the methods of deuterium exchange and deuterium labeling, since they can directly provide locations of membrane components. In addition, the methods of contrast variation with H2O-D2O mixtures, swelling, direct scaling of data from deuterium-labeled structures, and magnetic orientation have provided useful information in neutron diffraction analyses of membrane structures. Although this field is only in its infancy, it clearly has very encouraging prospects.
本文描述并展示了用于生物膜和膜成分中子衍射研究的方法和技术。其中最重要的是氘交换和氘标记方法，因为它们可以直接提供膜成分的位置。此外，利用H2O-D2O混合物进行对比度变化、溶胀、对氘标记结构的数据直接缩放以及磁取向等方法，在膜结构的中子衍射分析中提供了有用信息。尽管该领域尚处于起步阶段，但显然有着非常令人鼓舞的前景。
