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取向多层膜层状中子衍射分析的直接方法:一种差分帕特森反卷积方法。

Direct methods for the analysis of lamellar neutron diffraction from oriented multilayers: a difference Patterson deconvolution approach.

作者信息

Blasie J K, Schoenborn B P, Zaccai G

出版信息

Brookhaven Symp Biol. 1976 May(27):III58-III67.

PMID:963575
Abstract

We have described here a direct method for the solution of the phase problem for lamellar neutron diffraction from hydrated oriented multilayers of model membranes. As a result, the neutron-scattering profiles of such model membranes can be uniquely determined. We note that such methods can easily be extended to cases in which the thickness of the water layer w is somewhat greater than d/2 as long as it is substantially less than d [e.g., w less than 3d/4 would suffice, since deltaP(-d/4 less than or equal to x less than or equal to d/4) would contain only contributions from see article without overlapping contributions from see articles and to cases in which isotopic labeling is used instead of partial H2O-D2O exchange.

摘要

我们在此描述了一种直接求解来自模型膜水合取向多层膜的层状中子衍射相位问题的方法。结果,此类模型膜的中子散射轮廓能够被唯一确定。我们注意到,只要水层厚度w实质上小于d,这些方法就能轻松扩展到水层厚度w略大于d/2的情况[例如,w小于3d/4就足够了,因为δP(-d/4≤x≤d/4)将仅包含来自见文章的贡献,而不会与见文章的贡献重叠],并且能扩展到使用同位素标记而非部分H₂O-D₂O交换的情况。

相似文献

1
Direct methods for the analysis of lamellar neutron diffraction from oriented multilayers: a difference Patterson deconvolution approach.取向多层膜层状中子衍射分析的直接方法:一种差分帕特森反卷积方法。
Brookhaven Symp Biol. 1976 May(27):III58-III67.
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Neutron diffraction studies of biological membranes and membrane components.生物膜及膜成分的中子衍射研究。
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Advantages of neutron scattering for biological structure analysis.
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Neutron scattering for the analysis of membranes.用于膜分析的中子散射
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Analysis of solvent structure in proteins using neutron D2O-H2O solvent maps: pattern of primary and secondary hydration of trypsin.利用中子D2O-H2O溶剂图谱分析蛋白质中的溶剂结构:胰蛋白酶的一级和二级水合模式
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J Membr Biol. 2011 Jan;239(1-2):63-71. doi: 10.1007/s00232-010-9326-6. Epub 2010 Dec 14.
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Structure and hydration of membranes embedded with voltage-sensing domains.嵌入电压感应域的膜的结构和水合作用。
Nature. 2009 Nov 26;462(7272):473-9. doi: 10.1038/nature08542.
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Hydration of POPC bilayers studied by 1H-PFG-MAS-NOESY and neutron diffraction.
通过¹H-PFG-MAS-NOESY和中子衍射研究的POPC双层膜的水合作用。
Eur Biophys J. 2007 Apr;36(4-5):281-91. doi: 10.1007/s00249-007-0142-6. Epub 2007 Mar 1.
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Biological membrane structure as "seen" by X-ray and neutron diffraction techniques.通过X射线和中子衍射技术“观察”到的生物膜结构。
J Membr Biol. 1985;86(1):1-7. doi: 10.1007/BF01871604.