Ueda T, Ichikawa H, Igarashi O
Tokyo Metropolitan Research Laboratory of Public Health, Japan.
J Nutr Sci Vitaminol (Tokyo). 1993 Jun;39(3):207-19. doi: 10.3177/jnsv.39.207.
The alpha-tocopherol stereoisomers in biological specimens were investigated using high-performance liquid chromatography. All-rac-alpha-Toc acetate was separated into four peaks (peak area ratio: 4:2:1:1) by Chiralpak OP(+)HPLC. 2R-isomers constituted the first peak and 2S-isomers were separated into three peaks (peak area ratio: 2:1:1). 2-Ambo-alpha-Toc acetate was completely separated into RRR- and SRR-alpha-Toc acetate by this method. The present HPLC method was used for the separation of all-rac-alpha-Toc in blood and tissues of rat. The analytical recoveries of RRR- and SRR-alpha-Toc acetate added to blood and tissues were 90.5-98.2% for RRR-alpha-Toc and 93.7-100.5% for SRR-alpha-Toc. The distribution of alpha-Toc stereoisomers in the blood and tissues from rats administered all-rac-alpha-Toc acetate was investigated by HPLC. The concentrations of the 2R-isomers in the blood and tissues were markedly higher than the concentrations of the 2S-isomers, and the levels of alpha-Toc stereoisomers showed marked differences between blood and tissues.
采用高效液相色谱法对生物样品中的α-生育酚立体异构体进行了研究。消旋-α-生育酚醋酸酯通过Chiralpak OP(+)高效液相色谱法分离为四个峰（峰面积比为4:2:1:1）。2R-异构体构成第一个峰，2S-异构体分离为三个峰（峰面积比为2:1:1）。通过该方法，2-消旋-α-生育酚醋酸酯被完全分离为RRR-α-生育酚醋酸酯和SRR-α-生育酚醋酸酯。本高效液相色谱法用于大鼠血液和组织中消旋-α-生育酚的分离。添加到血液和组织中的RRR-α-生育酚醋酸酯和SRR-α-生育酚醋酸酯的分析回收率，RRR-α-生育酚为90.5-98.2%，SRR-α-生育酚为93.7-100.5%。通过高效液相色谱法研究了给予消旋-α-生育酚醋酸酯的大鼠血液和组织中α-生育酚立体异构体的分布。血液和组织中2R-异构体的浓度明显高于2S-异构体的浓度，并且α-生育酚立体异构体的水平在血液和组织之间存在显著差异。
