Fontbonne A, Badinand F
Centre d'Etudes en Reproduction Canine Assistée (CERCA), Ecole Nationale Vétérinaire, Maisons-Alfort, France.
J Reprod Fertil Suppl. 1993;47:325-7.
A total of 57 bitches of various breeds were inseminated with frozen semen [the extender used being Tris-fructose-egg yolk-6.4% (v/v) glycerol] under controlled conditions. The optimum time of insemination was determined using vaginal smears and progesterone assays. Of 38 bitches inseminated one to three times in the vagina using a technique employing a commercial intravaginal pipette, 20 (52.6%) became pregnant, with a mean litter size of 4.2. Of 19 bitches inseminated one to three times using a transcervical technique, the conception rate was 73.6% with a mean litter size of 5.5. These data show, for the freezing method used, that the results of artificial insemination with frozen semen in the bitch are better with intrauterine than with intravaginal deposition.
在可控条件下，用冷冻精液（稀释剂为含6.4%（v/v）甘油的Tris-果糖-蛋黄）对57只不同品种的母犬进行授精。通过阴道涂片和孕酮检测确定最佳授精时间。使用商用阴道内移液器技术在阴道内对38只母犬进行一至三次授精，其中20只（52.6%）怀孕，平均产仔数为4.2。使用经宫颈技术对19只母犬进行一至三次授精，受孕率为73.6%，平均产仔数为5.5。这些数据表明，就所采用的冷冻方法而言，母犬冷冻精液人工授精采用子宫内输精比阴道内输精效果更好。
