Obata H, Horiuchi H, Dobashi Y, Oka T, Sawa M, Machinami R
Department of Pathology, University of Tokyo School of Medicine, Japan.
Jpn J Ophthalmol. 1993;37(2):113-21.
Immunohistochemical localization of epidermal growth factor (EGF) in human main and accessory lacrimal glands has been investigated using monoclonal and polyclonal antibodies. Positive stain of EGF was localized in the cytoplasm of the acinar cells and the epithelial cells of the intra- and interlobular ducts of the main lacrimal glands, including both the palpebral and orbital lobes, and of the accessory lacrimal glands (gland of Krause, gland of Wolfring) as well. Meibomian glands showed weak immunoreactivity. Positive stain in the conjunctival epithelium including goblet cells was not observed. These results suggest that the mature form of EGF is produced in both the main and accessory human lacrimal glands and secreted into the tear fluid as a constant and crucial component.
利用单克隆抗体和多克隆抗体对人主泪腺和副泪腺中表皮生长因子（EGF）进行了免疫组织化学定位研究。EGF的阳性染色定位于主泪腺（包括睑叶和眶叶）以及副泪腺（克劳斯腺、沃尔夫林腺）的腺泡细胞和小叶内及小叶间导管的上皮细胞的细胞质中。睑板腺显示出弱免疫反应性。未观察到包括杯状细胞在内的结膜上皮有阳性染色。这些结果表明，成熟形式的EGF在人主泪腺和副泪腺中均有产生，并作为一种持续且关键的成分分泌到泪液中。
