Orita H, Fukazawa M, Inui K, Hirooka S, Uchino H, Fukui K, Kohi M, Washio M
Second Department of Surgery, Yamagata University School of Medicine, Japan.
Kyobu Geka. 1993 Oct;46(11):947-9.
We evaluated the functional and biochemical effects of prostaglandin I2 analogue (PGI-A) on cardiac myocytes incubated under hypothermic conditions. Myocytes were isolated from neonatal rat ventricles and cultured for 4 days. Then, myocytes (12.5 x 10(5) myocytes/flask) were incubated at 4 degrees C for 24 hrs in media with PGI-A as follows; O M PGI-A (group C: control), 10(-9) M (group P1), 10(-8) M (group P2), 10(-7) M (group P3), 10(-6) M (group P4). After hypothermic incubation, CPK and LDH were measured. The myocytes were then cultured for 24 hrs at 37 degrees C to evaluate the recovery of myocyte beating rate. For the beating rate, group P2 showed significantly increased recovery compared to the control (P2: 47.9, p < 0.025, C:18.1 percent of control; ie, beating rate prior to hypothermic incubation). The release of CPK and LDH was significantly suppressed in group P2 compared to the control (P2:57.7, p < 0.05, 275.1, p < 0.025; C:96.8 mIU/flask, 439.6 mIU/flask, respectively). In conclusion, prostaglandin I2 has direct cytoprotective characteristics for immature myocytes that may be suitable for cardiac preservation.
我们评估了前列腺素I2类似物（PGI-A）对在低温条件下培养的心肌细胞的功能和生化作用。从新生大鼠心室分离心肌细胞并培养4天。然后，将心肌细胞（12.5×10⁵个心肌细胞/培养瓶）在4℃下于含有PGI-A的培养基中孵育24小时，具体如下：0 M PGI-A（C组：对照组）、10⁻⁹ M（P1组）、10⁻⁸ M（P2组）、10⁻⁷ M（P3组）、10⁻⁶ M（P4组）。低温孵育后，测量肌酸磷酸激酶（CPK）和乳酸脱氢酶（LDH）。然后将心肌细胞在37℃下培养24小时，以评估心肌细胞搏动率的恢复情况。对于搏动率，与对照组相比，P2组显示出显著提高的恢复率（P2组：47.9，p<0.025，C组：为对照组的18.1%；即低温孵育前的搏动率）。与对照组相比，P2组中CPK和LDH的释放受到显著抑制（P2组：57.7，p<0.05，275.1，p<0.025；C组：分别为96.8 mIU/培养瓶、439.6 mIU/培养瓶）。总之，前列腺素I2对未成熟心肌细胞具有直接的细胞保护特性，这可能适用于心脏保存。
