Oliveira M M, Rocha E D, Rondinelli E, Arnholdt A V, Scharfstein J
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
Mol Cell Biochem. 1993 Jul 21;124(2):91-9. doi: 10.1007/BF00929200.
Fetal calf serum (FCS), which is mitogenic for the pathogenic protozoa T. cruzi, inhibits cAMP production in basal and forskolin-stimulated epimastigotes. It also activates phosphoinositides hydrolysis yielding diacylglycerol and inositol phosphates (Ins-P). Ins-P production is enhanced by AlF4-, GTP or beta-gamma-methylene-GTP, thus implying G proteins mediation in the phenomenon. An enzyme with phospholipase C activity which may be involved in the phospholipid metabolism was partially characterized.
胎牛血清（FCS）对致病性原生动物克氏锥虫具有促有丝分裂作用，它能抑制基础状态下以及福斯高林刺激的前鞭毛体中cAMP的产生。它还能激活磷酸肌醇水解，产生二酰甘油和肌醇磷酸（Ins-P）。AlF4-、GTP或β-γ-亚甲基-GTP可增强Ins-P的产生，因此表明该现象中有G蛋白介导。一种可能参与磷脂代谢的具有磷脂酶C活性的酶已得到部分表征。
