Oliveira M M, Antunes A, De Mello F G
Mol Biochem Parasitol. 1984 Apr;11:283-92. doi: 10.1016/0166-6851(84)90072-0.
Intracellular levels of cAMP in Trypanosoma cruzi epimastigotes were determined in the presence of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, and in the presence of the adrenergic ligand isoproterenol. An increase in the levels of cAMP was observed when those drugs were added. This effect was more pronounced when the cells were in the lag phase of growth. Phosphodiesterase inhibitors, catecholamines and dibutyryl cAMP inhibited proliferation of the organisms, as well as [3H]thymidine incorporation in in vitro experiments. These findings strongly suggest that cAMP plays a role in the control of growth of T. cruzi. The effects of adrenergic ligands in increasing cAMP levels and inhibiting growth could be reversed by beta-adrenergic antagonists, suggesting the presence of a receptor-mediated adenylate cyclase system in T. cruzi. Cholinergic ligands had no effect.
在磷酸二酯酶抑制剂3-异丁基-1-甲基黄嘌呤存在的情况下，以及在肾上腺素能配体异丙肾上腺素存在的情况下，测定了克氏锥虫前鞭毛体中的细胞内cAMP水平。添加这些药物后，观察到cAMP水平升高。当细胞处于生长延迟期时，这种效应更为明显。在体外实验中，磷酸二酯酶抑制剂、儿茶酚胺和二丁酰cAMP抑制了生物体的增殖以及[3H]胸腺嘧啶核苷的掺入。这些发现强烈表明，cAMP在克氏锥虫生长控制中发挥作用。肾上腺素能配体增加cAMP水平和抑制生长的作用可被β-肾上腺素能拮抗剂逆转，这表明克氏锥虫中存在受体介导的腺苷酸环化酶系统。胆碱能配体没有作用。
