Dusseau J Y, Chapalain J C, Rouby Y, Reverdy M E, Bartoli M
Laboratoire de Biologie Médicale, Hôpital d'Instruction des Armées Desgenettes, Lyon, France.
Pathol Biol (Paris). 1993 Apr;41(4):349-57.
The authors studied 5 disinfectants containing aldehyde and quaternary ammonium against 108 hospital strains. The minimal bactericidal activities (MBC) were determined with a microdilution method and the dilution-neutralization method (AFNOR T 72-150). The microdilution method results were validated using the MBCs of four reference strains tested with the dilution-neutralization method (AFNOR T 72-150). 3 disinfectants proved to be valuable. 1 disinfectant was valuable but with a MBC toward all bacteria only a dilution away from the dilution of use. 1 disinfectant was determined as unsatisfactory as 9.5 p. cent of all strains proved resistant toward it. All disinfectants matched AFNOR standards; but those specifications did not match toward hospital strains. Such an evaluation using a simple and reliable method should be of use.
作者研究了5种含醛和季铵盐的消毒剂对108株医院菌株的作用。采用微量稀释法和稀释中和法（法国标准化协会T 72 - 150）测定最小杀菌活性（MBC）。用稀释中和法（法国标准化协会T 72 - 150）检测的4株参考菌株的MBC对微量稀释法的结果进行验证。3种消毒剂被证明是有价值的。1种消毒剂有价值，但对所有细菌的MBC仅比使用稀释度低一个稀释倍数。1种消毒剂被判定为不合格，因为9.5%的菌株对其耐药。所有消毒剂均符合法国标准化协会标准；但这些规格与医院菌株不匹配。使用简单可靠的方法进行这样的评估应该会有用。
