Chantefort A, Lellouche M
Pathol Biol (Paris). 1984 Jun;32(5 Pt 2):623-7.
In this collaborative study, 23 French laboratories measured the bactericidal activity of the same disinfectant, a phenolic compound, on 3 bacterial strains (P. aeruginosa, S. aureus, and M. smegmatis), by AFNOR standards NF T 72-150 (dilution-neutralization method) and NF T 72-151 (membrane filtration method). Minimal bactericidal concentrations determined by both methods were identical or very similar (deviation by one dilution only). Thus, both standard methods produce the same results. Only three laboratories gave erroneous results. The twenty others found bactericidal concentrations which can be considered as correct (between half and double of calculated mean) in 80 to 95% of cases, depending on methods and strains. It may be estimated that 8 laboratories out of 10 master AFNOR techniques for evaluation of antiseptics and disinfectants.
在这项合作研究中，23个法国实验室按照法国标准化协会标准NF T 72 - 150（稀释中和法）和NF T 72 - 151（膜过滤法），测定了同一种消毒剂（一种酚类化合物）对3种细菌菌株（铜绿假单胞菌、金黄色葡萄球菌和耻垢分枝杆菌）的杀菌活性。通过这两种方法测定的最低杀菌浓度相同或非常相似（仅相差一个稀释度）。因此，这两种标准方法产生相同的结果。只有三个实验室给出了错误的结果。另外二十个实验室发现，根据方法和菌株不同，在80%至95%的情况下，杀菌浓度可被视为正确（在计算出的平均值的一半至两倍之间）。据估计，每10个实验室中有8个掌握了法国标准化协会评估防腐剂和消毒剂的技术。
