Dean T P, Dai Y, Shute J K, Church M K, Warner J O
Child Health, University of Southampton, England.
Pediatr Res. 1993 Aug;34(2):159-61. doi: 10.1203/00006450-199308000-00010.
Concurrent pulmonary inflammation and neutrophil infiltration are characteristic of children with cystic fibrosis (CF). The production of the major neutrophil chemotactic cytokine IL-8 by alveolar macrophages or other cells could be of great importance in the pathology of acute lung disease, but its role in the persistent lung inflammation characteristic of CF has not been evaluated. In this study, we have measured, by ELISA, the concentration of IL-8 in sputum, bronchoalveolar lavage, and sera specimens obtained from children with CF. For comparison, IL-8 in bronchoalveolar lavage obtained from asthmatic patients and from non-CF children with or without lung infection and in sera from age-matched controls was measured. High levels of IL-8 were measured in sputum (mean = 2952 pM) and in bronchoalveolar lavage (mean = 6624 pM) from CF patients. In both cases, there was a significant correlation between clinical status (Schwachman score) and IL-8 levels. This was not true for IL-8 levels measured in sera, which nevertheless were significantly higher in CF patients (p = 0.0001) than in normal controls in the over-10-y age group.
并发肺部炎症和中性粒细胞浸润是囊性纤维化（CF）患儿的特征。肺泡巨噬细胞或其他细胞产生主要的中性粒细胞趋化细胞因子白细胞介素-8（IL-8）在急性肺部疾病的病理过程中可能极为重要，但其在CF特征性的持续性肺部炎症中的作用尚未得到评估。在本研究中，我们通过酶联免疫吸附测定法（ELISA）测量了从CF患儿获取的痰液、支气管肺泡灌洗样本和血清样本中IL-8的浓度。为作比较，我们测量了哮喘患者、有或无肺部感染的非CF患儿的支气管肺泡灌洗样本中的IL-8以及年龄匹配对照组血清中的IL-8。在CF患者的痰液（平均 = 2952皮摩尔）和支气管肺泡灌洗样本（平均 = 6624皮摩尔）中检测到高水平的IL-8。在这两种情况下，临床状况（施瓦曼评分）与IL-8水平之间均存在显著相关性。血清中检测到的IL-8水平情况并非如此，不过在10岁以上年龄组中，CF患者血清中的IL-8水平显著高于正常对照组（p = 0.0001）。
