Morgan R, Edge M, Colman A
School of Biochemistry, University of Birmingham, UK.
Nucleic Acids Res. 1993 Sep 25;21(19):4615-20. doi: 10.1093/nar/21.19.4615.
Previously, antisense oligodeoxyribonucleotides (oligos) have been used to ablate specific mRNAs from the maternal RNA pool of Xenopus laevis oocytes. However, this strategy is limited by the dose of oligo which can be used and the fact that 100% cleavage of the target RNA is rare. Further, non-specific cleavage of other RNAs can also occur. We demonstrate that the use of several oligos against the histone H4 RNA results in a marked improvement in the efficiency of target degradation, due to synergistic action between oligos and the existence of RNA in at least two different secondary structures. We show, by using a set of overlapping oligos complementary to the entire H4 RNA, that the amount of oligo required for efficient target ablation is greatly lowered and non-specific effects are reduced.
此前，反义寡脱氧核糖核苷酸（oligos）已被用于从非洲爪蟾卵母细胞的母源RNA池中消除特定的mRNA。然而，该策略受到可使用的oligo剂量以及靶RNA很少能被100%切割这一事实的限制。此外，其他RNA的非特异性切割也可能发生。我们证明，由于oligos之间的协同作用以及RNA至少存在两种不同二级结构，使用几种针对组蛋白H4 RNA的oligos可显著提高靶标降解效率。我们通过使用一组与整个H4 RNA互补的重叠oligos表明，有效消除靶标所需的oligo量大大降低，非特异性效应也减少了。
