Fiedorowicz M, Galindo J R, Julliard M, Mannoni P, Chanon M
Laboratoire d'Activation, Mécanime, Modélisation Moléculaire: AMP-URA CNRS 1411, Faculté des Sciences Saint Jérôme, Marseille, France.
Photochem Photobiol. 1993 Sep;58(3):356-61. doi: 10.1111/j.1751-1097.1993.tb09574.x.
Photodynamic induced cytotoxicity by Victoria blue BO (VB-BO), merocyanine 540 (MC540), Nile blue A (NB) and 4-tetrasulfonatophenyl-porphyrin (4-TSPP) has been studied on two human leukemic cell lines: K-562 and TF-1. Cells were incubated with dyes and irradiated with different doses of white light. Cell survival was assessed by propidium iodide (PI) staining using flow cytometry analysis. Concentrations of 5 x 10(-8) M VB-BO were found to kill 75% of cells, and a concentration of 1 x 10(-7) M induced more than 99% of cell killing. To obtain the same cytotoxic level, the presence of 2.6 x 10(-5) M of MC540 during irradiation was needed. Under the conditions used, NB was ineffective as a photosensitizer, although uptake studies showed that this dye was taken by the cells in much greater amounts than any other studied dye. Cell cycle distribution of TF-1 cells, surviving MC540 or VB-BO photosensitization has been studied by flow cytometry analysis after staining with Hoechst 33342 and PI. It was found that cells in G1 phase were slightly more resistant toward MC540- and VB-BO-mediated photosensitization than cells in other phases of the cell cycle.
研究了维多利亚蓝BO（VB-BO）、部花青540（MC540）、尼罗蓝A（NB）和4-四磺酸苯基卟啉（4-TSPP）对两种人类白血病细胞系K-562和TF-1的光动力诱导细胞毒性。细胞与染料孵育后，用不同剂量的白光照射。通过碘化丙啶（PI）染色和流式细胞术分析评估细胞存活率。发现5×10⁻⁸ M的VB-BO浓度可杀死75%的细胞，而1×10⁻⁷ M的浓度可诱导超过99%的细胞死亡。为了达到相同的细胞毒性水平，照射期间需要存在2.6×10⁻⁵ M的MC540。在所使用的条件下，NB作为光敏剂无效，尽管摄取研究表明该染料被细胞摄取的量比其他任何研究的染料都要多得多。在用Hoechst 33342和PI染色后，通过流式细胞术分析研究了经MC540或VB-BO光敏化后存活的TF-1细胞的细胞周期分布。发现G1期的细胞对MC540和VB-BO介导的光敏化的抗性略高于细胞周期其他阶段的细胞。
