Hedreyda C T, Lee K K, Krause D C
Department of Microbiology, University of Georgia, Athens 30602.
Plasmid. 1993 Sep;30(2):170-5. doi: 10.1006/plas.1993.1047.
Mycoplasma pneumoniae was transformed with the Staphylococcus aureus transposon Tn4001 by electroporation. A transformation frequency of 10(-3) to 10(-5)/colony-forming unit was observed using 30.0 microgram plasmid DNA and 10(7)-10(8) M. pneumoniae colony-forming units. DNA hybridization analyses using standard and pulsed field agarose gel electrophoresis confirmed chromosomal insertion of the transposon, apparently by a transpositional mechanism into random sites. These studies demonstrate the functionality of Tn4001 in M. pneumoniae and suggest its potential as a genetic tool in this mycoplasma.
通过电穿孔法用金黄色葡萄球菌转座子Tn4001转化肺炎支原体。使用30.0微克质粒DNA和10⁷ - 10⁸个肺炎支原体菌落形成单位时，观察到转化频率为10⁻³至10⁻⁵/菌落形成单位。使用标准和脉冲场琼脂糖凝胶电泳进行的DNA杂交分析证实了转座子的染色体插入，显然是通过转座机制插入到随机位点。这些研究证明了Tn4001在肺炎支原体中的功能，并表明其作为该支原体遗传工具的潜力。
