用单链Tn4001 DNA对柔膜菌纲进行转化。

Transformation of Mollicutes with single-stranded Tn4001 DNA.

作者信息

Kapke P A, Knudtson K L, Minion F C

机构信息

Department of Microbiology, Immunology and Preventive Medicine, Iowa State University, Ames 50011.

出版信息

Plasmid. 1994 Jul;32(1):85-8. doi: 10.1006/plas.1994.1048.

DOI:10.1006/plas.1994.1048

PMID:7991677

Abstract

Acholeplasma oculi ISM1499 and Mycoplasma gallisepticum were transformed with single-stranded and double-stranded plasmids containing Tn4001. The transposon mobilized to the chromosome using both single-stranded and double-stranded DNA at the same frequency. M. gallisepticum transformed at a 2 log lower frequency than did A. oculi ISM1499. Restriction enzyme digestion of single-stranded DNA indicated homologous base pairing in the inverted repeat regions, which could account for the transpositional activity of single-stranded DNA.

摘要

用含有Tn4001的单链和双链质粒转化眼无胆甾原体ISM1499和鸡毒支原体。转座子利用单链和双链DNA以相同频率转移到染色体上。鸡毒支原体的转化频率比眼无胆甾原体ISM1499低2个对数。单链DNA的限制性酶切表明在反向重复区域存在同源碱基配对，这可以解释单链DNA的转座活性。