Brzobohatý B, Moore I, Kristoffersen P, Bako L, Campos N, Schell J, Palme K
Max-Planck Institut für Züchtungsforschung, Köln Federal Republic of Germany.
Science. 1993 Nov 12;262(5136):1051-4. doi: 10.1126/science.8235622.
A beta-glucoside encoded by a cloned Zea mays complementary DNA (Zm-p60.1) cleaved the biologically inactive hormone conjugates cytokinin-O-glucosides and kinetin-N3-glucoside, releasing active cytokinin. Tobacco protoplasts that transiently expressed Zm-p60.1 could use the inactive cytokinin glucosides to initiate cell division. The ability of protoplasts to sustain growth in response to cytokinin glucosides persisted indefinitely after the likely disappearance of the expression vector. In the roots of maize seedlings, Zm-p60.1 was localized to the meristematic cells and may function in vivo to supply the developing maize embryo with active cytokinin.
由克隆的玉米互补DNA（Zm-p60.1）编码的一种β-葡萄糖苷可切割无生物活性的激素共轭物细胞分裂素-O-葡萄糖苷和激动素-N3-葡萄糖苷，释放出活性细胞分裂素。瞬时表达Zm-p60.1的烟草原生质体能够利用无活性的细胞分裂素葡萄糖苷启动细胞分裂。在表达载体可能消失后，原生质体对细胞分裂素葡萄糖苷作出反应以维持生长的能力会无限期持续。在玉米幼苗的根中，Zm-p60.1定位于分生细胞，可能在体内发挥作用，为发育中的玉米胚提供活性细胞分裂素。
