Yang Haizhu, Yao Xin, Wu Weijiao, He Ailing, Ma Chao, Yang Sanwei, Ruan Jingjun
College of Agriculture, Guizhou University, Guiyang, Guizhou, China.
BMC Plant Biol. 2024 Dec 18;24(1):1183. doi: 10.1186/s12870-024-05919-3.
The β-glucosidases (BGLU) of glycoside hydrolase family 1 hydrolyze the glycosidic bond to release β-D-glucose and related ligands, which are widely involved in important physiological processes in plants. Genome-wide analysis of the BGLU genes in the model crops Arabidopsis thaliana and Oryza sativa revealed that they are functionally diverse. In contrast, the BGLU gene family in Tartary buckwheat remains unclear.
This study identified the FtBGLU gene family based on Tartary buckwheat genomic data and analyzed the biological function of the FtBGLU gene using bioinformatics methods and the expression pattern of the gene using fluorescence quantitative PCR. The results showed that 39 BGLU genes were identified in Tartary buckwheat, which were classified into 10 subfamilies and one unclassified group. They were unevenly distributed on 10 chromosomes, and seven tandem duplication events involving 19 FtBGLU genes were observed, which mainly occurred in subfamily II. Their physicochemical properties are highly variable; however, they have relatively conserved exon-intron structures and high sequence homology in the subfamily, and most of the FtBGLUs contain conserved motifs, among which the expression products FtBGLU1, FtBGLU17, FtBGLU19, FtBGLU21, FtBGLU22, and FtBGLU28 have no β-glucosidase activity. Additionally, we analyzed the tissue expression specificity of 10 FtBGLU genes during Tartary buckwheat growth and development and their expression patterns under adversity stress and hormone treatments. Revealing the important role of the BGLU gene family in Tartary buckwheat growth and development, as well as its response to adversity, provides strong support for further analysis of its regulatory mechanisms and functional applications. A total of 39 FtBGLU genes were identified. Bioinformatics analysis of the gene structure, evolutionary relationship, and expression pattern of the Fagopyrum tataricum BGLU gene family establishes a foundation for a better understanding and future research on the Tartary buckwheat BGLU gene family.
糖苷水解酶家族1的β-葡萄糖苷酶（BGLU）可水解糖苷键以释放β-D-葡萄糖及相关配体，其广泛参与植物的重要生理过程。对模式作物拟南芥和水稻中BGLU基因的全基因组分析表明，它们功能多样。相比之下，苦荞中BGLU基因家族尚不清楚。
本研究基于苦荞基因组数据鉴定了FtBGLU基因家族，并利用生物信息学方法分析了FtBGLU基因的生物学功能，通过荧光定量PCR分析了该基因的表达模式。结果表明，苦荞中鉴定出39个BGLU基因，分为10个亚家族和1个未分类组。它们不均匀地分布在10条染色体上，观察到涉及19个FtBGLU基因的7个串联重复事件，主要发生在亚家族II中。它们的理化性质高度可变；然而，它们具有相对保守的外显子-内含子结构，且在亚家族中具有较高的序列同源性，大多数FtBGLU含有保守基序，其中表达产物FtBGLU1、FtBGLU17、FtBGLU19、FtBGLU21、FtBGLU22和FtBGLU28没有β-葡萄糖苷酶活性。此外，我们分析了10个FtBGLU基因在苦荞生长发育过程中的组织表达特异性及其在逆境胁迫和激素处理下的表达模式。揭示BGLU基因家族在苦荞生长发育及其对逆境响应中的重要作用，为进一步分析其调控机制和功能应用提供了有力支持。共鉴定出39个FtBGLU基因。对苦荞BGLU基因家族的基因结构、进化关系和表达模式进行生物信息学分析，为更好地理解苦荞BGLU基因家族及未来研究奠定了基础。
