Carvalho M R, Krieger M A, Almeida E, Oelemann W, Shikanai-Yassuda M A, Ferreira A W, Pereira J B, Sáez-Alquézar A, Dorlhiac-Llacer P E, Chamone D F
Fundação Pró-Sangue Hemocentro de São Paulo, Brazil.
Transfusion. 1993 Oct;33(10):830-4. doi: 10.1046/j.1537-2995.1993.331094054620.x.
Blood transfusion is one of the principal routes of transmission of Chagas' disease, a major endemic disease in Latin America. Methods for blood screening are not accurate and may yield false results that lead to high social and economic costs. This study compares two methods of diagnosing Chagas' disease (indirect immunofluorescence and hemagglutination) and several enzyme-linked immunosorbent assays (ELISAs) with regard to specificity and sensitivity, by using human sera with known serologic and parasitologic characteristics, as well as samples with discrepant results on conventional serologic tests. An ELISA using recombinant antigens showed no cross-reactivity with sera that were positive for other diseases. All evaluated ELISAs performed well, and their use may lead to a reduction of more than 50 percent in the number of discordant sera. Further improvements are needed in view of the complexity of the serologic diagnosis of Chagas' disease.
输血是恰加斯病（拉丁美洲一种主要的地方病）的主要传播途径之一。血液筛查方法并不准确，可能会产生导致高昂社会和经济成本的错误结果。本研究通过使用具有已知血清学和寄生虫学特征的人血清以及在传统血清学检测中结果不一致的样本，比较了两种诊断恰加斯病的方法（间接免疫荧光法和血凝法）以及几种酶联免疫吸附测定（ELISA）在特异性和敏感性方面的差异。一种使用重组抗原的ELISA与其他疾病阳性血清无交叉反应。所有评估的ELISA表现良好，使用它们可能会使结果不一致的血清数量减少50%以上。鉴于恰加斯病血清学诊断的复杂性，仍需要进一步改进。
