Yanagida T, Harada Y, Ishijima A
Department of Biophysical Engineering, Osaka University, Japan.
Trends Biochem Sci. 1993 Sep;18(9):319-24. doi: 10.1016/0968-0004(93)90064-t.
Techniques have been recently developed that allow the direct observation of single actin filaments and their manipulation, using glass microneedles, in the nanometer range. Further development of these techniques has made possible the detection of subpiconewton-level forces of individual myosin heads. This in vitro motility model is sensitive in the submillisecond range and has allowed us to determine the force generation of an actomyosin motor directly at the molecular level. The results have led to a new conceptual framework for chemo-mechanical energy transduction in the molecular motor.
最近已经开发出了一些技术，这些技术能够使用玻璃微针在纳米范围内直接观察单个肌动蛋白丝并对其进行操控。这些技术的进一步发展使得检测单个肌球蛋白头部的亚皮牛顿级力成为可能。这种体外运动模型在亚毫秒范围内很敏感，使我们能够直接在分子水平上确定肌动球蛋白马达的力产生情况。这些结果为分子马达中的化学机械能转换带来了一个新的概念框架。
