Phai L D, Reuter G
Z Allg Mikrobiol. 1976;19(5):369-75.
Diphenylamine (DPA) causes an increased leakage of organic compounds with resting cells of Candida guilliermondii, Bacillus subtilis, Escherichia coli, and Staphylococcus aureus already after a treatment of 1 h. The leakage depends both on the concentration of DPA and the duration of the treatment. Moreover, DPA influences the composition of the leaked ninhydrine-positive compounds. After 20 h of treatment an increased release of mannan and ribose is observed in addition to the stronger leakage of ninhydrine positive compounds. With regard to the relation between structure and activity of DPA, the activity of 21 compounds was investigated. The two apolar phenyl groups as well as the secondary amino group being able to form ionic bounds are essential for maximal activity. A disturbance of the function of the cell membrane caused by DPA is discussed.
二苯胺（DPA）在处理1小时后，就会导致季也蒙念珠菌、枯草芽孢杆菌、大肠杆菌和金黄色葡萄球菌的静息细胞中有机化合物的泄漏增加。泄漏既取决于DPA的浓度，也取决于处理的持续时间。此外，DPA会影响泄漏的茚三酮阳性化合物的组成。处理20小时后，除了茚三酮阳性化合物的泄漏增强外，还观察到甘露聚糖和核糖的释放增加。关于DPA的结构与活性之间的关系，研究了21种化合物的活性。两个非极性苯基以及能够形成离子键的仲氨基对于最大活性至关重要。文中还讨论了DPA引起的细胞膜功能紊乱。
