动物凝固酶阳性葡萄球菌中一种异常细胞壁多糖的免疫化学分析。2. 基于化学和血清学研究的可能结构

Immunochemical analysis of an unusual cell wall polysaccharide from animal coagulase-positive staphylococci. 2. Probable structure based on chemical and serological studies.

作者信息

Endresen C, Grov A

出版信息

Acta Pathol Microbiol Scand B. 1976 Oct;84B(5):305-8. doi: 10.1111/j.1699-0463.1976.tb01942.x.

DOI:10.1111/j.1699-0463.1976.tb01942.x

Abstract

The teichoic acid of polysaccharide P (poly P) contains glycerol, glucose and phosphate. Hydrofluoric acid and alkali hydrolysates contain glycerol 1-phosphate, glycerol diphosphate, and glucose 1-phosphate, but no glucosyl-glycerol fragments. Glucose and the serological activity of poly P were destroyed by periodate oxidation. Interaction with concanavalin A showed that the glucose is in alpha-configuration and that the hydroxyl groups at positions 3, 4 and 6 are unsubstituted. Most probably, the poly P teichoic acid is a polymer containing a repeating unit in which glycerol 1-phosphate is attached to the 2-position on alpha-D-glucose 1-phosphate.

摘要

多糖P（聚P）的磷壁酸含有甘油、葡萄糖和磷酸盐。氢氟酸和碱水解产物含有甘油-1-磷酸、甘油二磷酸和葡萄糖-1-磷酸，但没有葡糖基甘油片段。高碘酸盐氧化破坏了葡萄糖和聚P的血清学活性。与伴刀豆球蛋白A的相互作用表明，葡萄糖呈α构型，且3、4和6位的羟基未被取代。聚P磷壁酸很可能是一种聚合物，其重复单元中甘油-1-磷酸连接到α-D-葡萄糖-1-磷酸的2位上。