Lunde M N, Powers K G
Ann Trop Med Parasitol. 1976 Sep;70(3):283-91. doi: 10.1080/00034983.1976.11687124.
Methods of preparing malaria antigen were compared. In one experiment a Ficoll density gradient was used to obtain a purified suspension of Plasmodium knowlesi schizonts from which antigen was prepared. Evaluation of methods such as freeze-thawing and the use of the French press following saponin lysis of schizonts demonstrated that a single freeze-thaw was the best method for obtaining the most antigen. A second experiment was done comparing the antigen prepared from schizont infected erythrocytes separated on a Ficoll density gradient with antigen prepared without separation of schizont infected erythrocytes on a Ficoll gradient. Saponin treatment of erythrocytes without the use of a Ficoll gradient followed by either freeze-thawing or the French press appeared to be the method of choice in preparing malaria IHA antigen.
对疟疾抗原的制备方法进行了比较。在一项实验中，使用菲可密度梯度从诺氏疟原虫裂殖体中获得纯化悬浮液，进而制备抗原。对诸如冻融法以及在裂殖体皂素裂解后使用法国压榨机等方法的评估表明，单次冻融是获得最多抗原的最佳方法。进行了第二项实验，比较了通过菲可密度梯度分离裂殖体感染红细胞制备的抗原与未通过菲可梯度分离裂殖体感染红细胞制备的抗原。在不使用菲可梯度的情况下对红细胞进行皂素处理，然后进行冻融或使用法国压榨机，这似乎是制备疟疾间接血凝试验（IHA）抗原的首选方法。
