Kowalski M L, Grzegorczyk J, Sliwinska-Kowalska M, Wojciechowska B, Rozniecka M, Rozniecki J
Department of Pulmonology and Allergology, Faculty of Medicine, Medical Academy, Lódź, Poland.
Allergy. 1993 Aug;48(6):409-14. doi: 10.1111/j.1398-9995.1993.tb00738.x.
In order to elucidate the mechanism responsible for infiltration of nasal mucosa by granulocytes, we tested neutrophil chemotactic activity (NCA) in nasal lavages, by the modified Boyden chamber method, in 16 patients with perennial allergic rhinitis (AR), six ASA-sensitive patients with chronic rhinosinusitis (CRS), and seven normal, nonatopic control subjects (NC). Nasal secretions from all three groups showed significant NCA (mean 157.1 +/- 54.0, 62.2 +/- 20.7, and 39.4 +/- 11.4% of FMLP chemotactic activity for AR, CRS, and NC subjects, respectively). Nasal secretions from patients with AR expressed significantly higher NCA (P < 0.02) than did secretions from NA patients. NCA was unchanged by heating at 56 degrees C for 60 min and was not susceptible to degradation by trypsin. Nasal challenge with Dermatophagoides pteronyssinus antigen induced clinical symptoms and resulted in significant increases in total protein and albumin concentrations in nasal lavages in AR patients, but failed to change the mean NCA activity for up to 40 min after the challenge. These results indicate that nasal secretions from both atopic and nonatopic patients express NCA, but its relation to allergic inflammation remains to be established.
为了阐明粒细胞浸润鼻黏膜的机制，我们采用改良的Boyden小室法，检测了16例常年性变应性鼻炎（AR）患者、6例对阿司匹林敏感的慢性鼻-鼻窦炎（CRS）患者及7名正常非特应性对照者（NC）鼻灌洗液中的中性粒细胞趋化活性（NCA）。三组患者的鼻分泌物均显示出显著的NCA（AR组、CRS组和NC组的NCA分别为FMLP趋化活性的157.1±54.0%、62.2±20.7%和39.4±11.4%）。AR患者的鼻分泌物中NCA显著高于非AR患者（P<0.02）。NCA在56℃加热60分钟后无变化，且不易被胰蛋白酶降解。用尘螨抗原进行鼻腔激发可诱发AR患者的临床症状，并导致鼻灌洗液中总蛋白和白蛋白浓度显著升高，但激发后长达40分钟内，平均NCA活性未发生变化。这些结果表明，特应性和非特应性患者的鼻分泌物均表达NCA，但其与变应性炎症的关系尚待确定。
