Sung A M, Nordskog A W, Lamont S J, Warner C M
Department of Biochemistry and Biophysics, Iowa State University, Ames 50011.
Anim Genet. 1993 Aug;24(4):227-33. doi: 10.1111/j.1365-2052.1993.tb00304.x.
The chicken major histocompatibility complex (MHC), the B complex, is being intensively analysed at the DNA level. To further probe the molecular structure of chicken MHC class II genes, cDNA clones coding for chicken MHC class II (B-L) beta chain molecules were isolated from an inbred G-B2 Leghorn chicken spleen and liver. Twenty-nine cDNA clones were isolated from the spleen and eight cDNA clones were isolated from the liver. Based on restriction maps, most clones could be clustered into one family of genes. Four cDNA clones were sequenced (S7, S10 and S19 from the spleen and L1, which was identical to S19, from the liver). Complete amino acid sequences of B-L beta chain molecules were predicted from the nucleotide sequences of the cDNA clones. Although both the nature and the location of the conserved residues were similar in chicken and mammalian sequences, some species-specific differences were found, suggesting that the structures of the B-L molecules of this haplotype are similar, but not identical, to their mammalian counterparts.
鸡的主要组织相容性复合体（MHC），即B复合体，正在DNA水平上进行深入分析。为了进一步探究鸡MHC II类基因的分子结构，从近交系G-B2来亨鸡的脾脏和肝脏中分离出编码鸡MHC II类（B-L）β链分子的cDNA克隆。从脾脏中分离出29个cDNA克隆，从肝脏中分离出8个cDNA克隆。根据限制性图谱，大多数克隆可聚为一个基因家族。对4个cDNA克隆进行了测序（脾脏中的S7、S10和S19，以及肝脏中的L1，L1与S19相同）。从cDNA克隆的核苷酸序列预测了B-Lβ链分子的完整氨基酸序列。尽管鸡和哺乳动物序列中保守残基的性质和位置相似，但也发现了一些物种特异性差异，这表明该单倍型B-L分子的结构与其哺乳动物对应物相似，但并不相同。
