Cízová D, Gábrisová E, Stratil A, Hojný J
Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Libĕchov.
Anim Genet. 1993 Aug;24(4):315-8. doi: 10.1111/j.1365-2052.1993.tb00320.x.
A further alpha-protease inhibitor system, PI4, was detected in porcine sera using either 2D agarose gel, pH 5.0-PAGE, pH 9.0, or 1D PAGE followed by immunoblotting with rabbit anti-porcine PI2 or PI3 antisera. PI4 inhibited chymotrypsin, but not trypsin. Seven allelic variants of PI4 were described. By haplotyping of alpha-protease inhibitor systems in 52 complete families it was shown that PI4 locus belongs to the PI gene cluster. The probable order of the PI loci was: PI1, PO1A, PI2, PI4, PI3.
利用二维琼脂糖凝胶、pH 5.0聚丙烯酰胺凝胶电泳、pH 9.0聚丙烯酰胺凝胶电泳或一维聚丙烯酰胺凝胶电泳，随后用兔抗猪PI2或PI3抗血清进行免疫印迹，在猪血清中检测到了另一种α-蛋白酶抑制剂系统PI4。PI4抑制胰凝乳蛋白酶，但不抑制胰蛋白酶。已描述了PI4的7个等位基因变体。通过对52个完整家系的α-蛋白酶抑制剂系统进行单倍型分析，结果表明PI4基因座属于PI基因簇。PI基因座的可能顺序为：PI1、PO1A、PI2、PI4、PI3。
