Robinson J A, Allen G K, Green E M, Garner H E, Loch W E, Walsh D M
Department of Veterinary Microbiology, College of Veterinary Medicine, University of Missouri-Columbia 65211.
Am J Vet Res. 1993 Sep;54(9):1411-4.
Serum interleukin-6 (IL-6) concentration was measured in 11 colostrum-fed (CF) and 8 colostrum-deprived (CD) 2- to 3-day-old foals after foals were infused with lipopolysaccharide (LPS; Escherichia coli O55:B5 endotoxin, 0.5 microgram/kg of body weight in sterile saline [0.9% NaCl] solution). Four CF and 2 CD foals were given saline solution alone. Serum IL-6 concentration was estimated by use of an in vitro proliferative bioassay, using the IL-6 dependent B.13.29 clone 9 cells. Interleukin-6 concentration increased in all LPS-infused foals, and geometric mean serum IL-6 concentration was significantly higher in CF than CD foals 30 and 90 minutes after infusion. Both LPS-infused groups had multiple spikes of mean IL-6 concentration that peaked at 120 minutes in CF foals and 150 minutes in CD foals. Results indicated that IL-6 is produced in neonatal foals in response to LPS infusion. Furthermore, colostrum deprivation resulted in longer times to peak mean serum IL-6 concentration and tended to reduce serum IL-6 concentration in neonatal foals.
在给2至3日龄的马驹静脉注射脂多糖（LPS；大肠杆菌O55:B5内毒素，溶于无菌生理盐水[0.9%氯化钠]溶液中，剂量为0.5微克/千克体重）后，测定了11头初乳喂养（CF）和8头未喂初乳（CD）的马驹的血清白细胞介素-6（IL-6）浓度。4头CF马驹和2头CD马驹仅给予生理盐水溶液。使用依赖IL-6的B.13.29克隆9细胞，通过体外增殖生物测定法估算血清IL-6浓度。所有注射LPS的马驹的白细胞介素-6浓度均升高，在注射后30分钟和90分钟时，CF马驹的血清IL-6几何平均浓度显著高于CD马驹。两个注射LPS的组的平均IL-6浓度均有多个峰值，CF马驹在120分钟时达到峰值，CD马驹在150分钟时达到峰值。结果表明，新生马驹在注射LPS后会产生IL-6。此外，初乳剥夺导致血清IL-6平均浓度达到峰值的时间延长，并倾向于降低新生马驹的血清IL-6浓度。
