Baskett A, Barton M H, Norton N, Anders B, Moore J N
Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
Am J Vet Res. 1997 Nov;58(11):1291-9.
To compare effects of a single dose of pentoxifylline (PTX), flunixin meglumine (FM), and their combination (FM/PTX) in a model of equine endotoxemia.
24 healthy horses, aged 2 to 15 years.
4 groups (n = 6/group) received 30 ng of Escherichia coli O55:B5 endotoxin/kg of body weight, i.v., over 30 minutes, and 1 of the following preparations 15 minutes before and 8 hours after endotoxin infusion: FM, 1.1 mg/kg; PTX, 8 mg/kg; FM/PTX, 1.1 mg of FM and 8 mg of PTX/kg; and saline solution bolus (ENDO). Clinical and hematologic variables were measured over 24 hours.
Compared with ENDO, FM given before endotoxin significantly reduced TxB2, and 6-keto-PGF1 concentrations, pulse, rectal temperature, and attitude score. Pentoxifylline given before endotoxin resulted in significantly higher 6-keto-PGF1 concentration at 1.5 hours and significantly lower PAI-1 activity at 12 hours. Tumor necrosis factor and IL-6 activities in horses given PTX alone were not significantly different from values in those given the saline bolus. FM/PTX induced effects similar to those of FM alone on endotoxin-induced changes in temperature and TxB2 concentration, and 6-keto-PGF1 concentration was significantly lower than that in horses of the ENDO group at 1 hour. In horses of the FM group, 6-keto-PGF1 concentration was significantly lower than that in horses of the ENDO group, from 0.5 hour to 2 hours. Horses of the FM and FM/PTX groups had significantly higher IL-6 activity at 1.5 and 2 hours than did horses of the PTX and ENDO groups; those of the FM and FM/PTX groups had significantly lower WBC count than did those of the PTX and ENDO groups.
FM/PTX may help offset deleterious hemodynamic effects of endotoxin more effectively than does either FM or PTX alone.
比较单剂量己酮可可碱（PTX）、氟尼辛葡甲胺（FM）及其组合（FM/PTX）在马内毒素血症模型中的作用。
24匹2至15岁的健康马。
4组（每组n = 6）在30分钟内静脉注射30 ng大肠杆菌O55:B5内毒素/千克体重，并在内毒素输注前15分钟和输注后8小时给予以下制剂之一：FM，1.1 mg/kg；PTX，8 mg/kg；FM/PTX，1.1 mg FM和8 mg PTX/kg；以及生理盐水推注（ENDO）。在24小时内测量临床和血液学变量。
与ENDO组相比，内毒素注射前给予FM可显著降低血栓素B2（TxB2）和6-酮-前列腺素F1（6-keto-PGF1）浓度、脉搏、直肠温度和态度评分。内毒素注射前给予己酮可可碱在1.5小时时导致6-酮-前列腺素F1浓度显著升高，在12小时时导致纤溶酶原激活物抑制剂-1（PAI-1）活性显著降低。单独给予PTX的马中肿瘤坏死因子和白细胞介素-6（IL-6）活性与给予生理盐水推注的马的值无显著差异。FM/PTX对内毒素诱导的体温和TxB2浓度变化产生的作用与单独给予FM相似，且在1小时时6-酮-前列腺素F1浓度显著低于ENDO组的马。在FM组的马中，从0.5小时至2小时，6-酮-前列腺素F1浓度显著低于ENDO组的马。FM组和FM/PTX组的马在1.5小时和2小时时的IL-6活性显著高于PTX组和ENDO组的马；FM组和FM/PTX组的马的白细胞计数显著低于PTX组和ENDO组的马。
与单独使用FM或PTX相比，FM/PTX可能更有效地帮助抵消内毒素的有害血流动力学作用。
