Robinson W E, Ryan D K, Wallace G T
Environmental Sciences Program, University of Massachusetts-Boston 02125.
Arch Environ Contam Toxicol. 1993 Nov;25(4):415-21. doi: 10.1007/BF00214329.
Ingested matter can have a significant effect on whole body metal concentration measurements in Mytilus edulis. Depuration of mussels in clean seawater for 36 h prior to dissection eliminates most of these contaminating gut contents. Depuration followed by metal analyses is the most direct method of determining mussel tissue metal bioburdens. After being transplanted into a plume of primary treated sewage effluent in Salem Harbor, Massachusetts for 32 days, Al, Cr, and Fe concentrations in depurated mussels were significantly lower than those determined for either non-depurated mussels or for depurated mussels to which fecal concentrations of Al, Cr, and Fe were added back in. Although mathematical methods developed by both Ouellette (1978) and Boehm et al. (1988) could be applied to non-depurated mussels in order to correct for errors associated with gut metal contamination, these indirect methods were not as reliable as depuration prior to analysis.
摄入物质对紫贻贝的全身金属浓度测量可能有显著影响。在解剖前将贻贝置于清洁海水中净化36小时，可去除大部分这些污染性的肠道内容物。净化后进行金属分析是确定贻贝组织金属生物负荷的最直接方法。在马萨诸塞州塞勒姆港被移植到经过一级处理的污水排放羽流中32天后，净化后的贻贝中铝、铬和铁的浓度显著低于未净化的贻贝或添加了铝、铬和铁粪便浓度的净化贻贝所测定的浓度。尽管Ouellette（1978年）和Boehm等人（1988年）开发的数学方法可应用于未净化的贻贝，以校正与肠道金属污染相关的误差，但这些间接方法不如分析前的净化可靠。
