Czarny A, Witkowska D, Mulczyk M
Laboratory of Medical Microbiology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław.
Arch Immunol Ther Exp (Warsz). 1993;41(2):153-7.
The ability of outer membrane proteins (OMP) of Shigella flexneri to induce tumor necrosis factor (TNF) and interleukin 6 (IL-6) production by spleen cells and macrophages of mice was investigated. Treatment of spleen cells with OMP resulted in the release of only traces of TNF activity. In contrast, macrophages treated with OMP produced moderate levels of TNF. OMP was also found to be an inducer of IL-6. Both spleen cells and macrophages, treated with OMP, were found to produce substantial levels of this cytokine. The effect of OMP on the release of TNF and IL-6 was dose and time dependent, maximal production being reached at 10 micrograms of OMP after 20 h. The ability of OMP to induce production of these cytokines may explain part of the previously described immunomodulatory effects of this preparation on the immune system.
研究了福氏志贺菌外膜蛋白（OMP）诱导小鼠脾细胞和巨噬细胞产生肿瘤坏死因子（TNF）和白细胞介素6（IL-6）的能力。用OMP处理脾细胞仅导致痕量TNF活性的释放。相比之下，用OMP处理的巨噬细胞产生中等水平的TNF。还发现OMP是IL-6的诱导剂。用OMP处理的脾细胞和巨噬细胞均产生大量这种细胞因子。OMP对TNF和IL-6释放的影响呈剂量和时间依赖性，20小时后10微克OMP时达到最大产量。OMP诱导这些细胞因子产生的能力可能解释了该制剂先前描述的对免疫系统的免疫调节作用的一部分。
