Yan L, Fridland G, Tseng J L, Desiderio D M
Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee, Memphis 38163.
Biochem Biophys Res Commun. 1993 Oct 29;196(2):521-6. doi: 10.1006/bbrc.1993.2281.
A new analytical system has been designed to characterize intermediate-sized precursor molecules from two different precursors-the preproenkephalin Abovine,1-263 precursor molecule, which produces methionine enkephalin and from the proopiomelanocortin, POMCbovine,1-265 precursor molecule, which produces beta-endorphin. A possible intermediate-sized precursor that leads to beta-endorphin was found in bovine pituitary by means of electrospray ionization mass spectrometry, in combination with gel permeation chromatography, reversed-phase high performance liquid chromatography, radioimmunoassay, trypsinolysis, and liquid secondary ion mass spectrometry.
一种新的分析系统已被设计用于表征来自两种不同前体的中等大小的前体分子——前脑啡肽原牛1 - 263前体分子，它产生甲硫氨酸脑啡肽；以及阿片促黑激素皮质素原牛1 - 265前体分子，它产生β-内啡肽。通过电喷雾电离质谱法，并结合凝胶渗透色谱法、反相高效液相色谱法、放射免疫测定法、胰蛋白酶消化法和液体二次离子质谱法，在牛垂体中发现了一种可能导致β-内啡肽产生的中等大小的前体。
