Faull K F, Feistner G J, Conklin K, Roepstorff P, Andrews P C
Department of Chemistry Biochemistry, University of California Los Angeles 90095, USA.
Neuropeptides. 1998 Aug;32(4):339-49. doi: 10.1016/s0143-4179(98)90057-9.
In-depth investigations, by high performance liquid chromatographic purification, radio-immunoassay, mass spectrometry, tandem mass spectrometry, Edman sequencing and limited C-terminal ladder sequencing, were prompted by mass spectrometric charting experiments which suggested that the amino acid sequences for rat gamma-lipotrophin and beta-endorphin require revision. The results for gamma-lipotrophin identify a histidine for glutamine substitution at position 12, and heterogeneity in the expressed protein presumably due to partial dehydration. Partial dehydration for acidic joining peptide, previously reported by Toney et al was corroborated. The results for beta-endorphin confirm the presence of alanine at position 26 and provide no evidence for the expression of multiple forms of the hormone.
通过高效液相色谱纯化、放射免疫测定、质谱分析、串联质谱分析、埃德曼测序和有限的C端阶梯测序进行的深入研究，是由质谱图谱实验引发的，这些实验表明大鼠γ-促脂素和β-内啡肽的氨基酸序列需要修订。γ-促脂素的结果确定了第12位谷氨酰胺被组氨酸取代，并且表达的蛋白质存在异质性，推测这是由于部分脱水所致。Toney等人先前报道的酸性连接肽的部分脱水得到了证实。β-内啡肽的结果证实了第26位存在丙氨酸，并且没有证据表明该激素存在多种形式的表达。
