鉴定一种由三螺旋区域广泛中断所代表的新型胶原链。

Identification of a novel collagen chain represented by extensive interruptions in the triple-helical region.

作者信息

Abe N, Muragaki Y, Yoshioka H, Inoue H, Ninomiya Y

机构信息

Department of Molecular Biology and Biochemistry, Okayama University Medical School, Japan.

出版信息

Biochem Biophys Res Commun. 1993 Oct 29;196(2):576-82. doi: 10.1006/bbrc.1993.2288.

DOI:10.1006/bbrc.1993.2288

PMID:8240330

Abstract

We have isolated mouse cDNA clones that encode a newly discovered collagenous polypeptide. Four overlapping cDNA clones contained an open reading frame of 1140 amino acid residues of collagenous and non-collagenous domains. The predicted polypeptide consists of 10 collagenous domains of various length that are interrupted by 11 noncollagenous domains. The overall structural arrangement differed significantly from reported collagen chains of 17 different types. Northern-blot analyses showed hybridization of the cDNA to 5.6kb and 4.8kb mRNA species in liver and kidney that are due to utilization of the two poly (A) signals.

摘要

我们已经分离出编码一种新发现的胶原多肽的小鼠cDNA克隆。四个重叠的cDNA克隆包含一个由1140个氨基酸残基组成的开放阅读框，这些残基分布在胶原结构域和非胶原结构域中。预测的多肽由10个不同长度的胶原结构域组成，这些结构域被11个非胶原结构域打断。其整体结构排列与已报道的17种不同类型的胶原链有显著差异。Northern印迹分析显示，由于使用了两个聚腺苷酸信号，该cDNA与肝脏和肾脏中5.6kb和4.8kb的mRNA种类发生杂交。